DNA

Part:BBa_K1971013

Designed by: KE GUAN YU   Group: iGEM16_BHU_China   (2016-10-20)


t7-ompt-mal85-t7 terminator

t7-ompt-mal85

Usage and Biology

Filamentous hemagglutinin (FHA) is a unique prokaryotic protein that promotes the interaction of the human pathogen Bordetella pertussis with host cells. Mal85 is a 1371bp fragment from this large 220 kDa protein and it’s proven to be a strong immunodominant domain. It’s recognized by the sera from patients with pertussis and sera from infants vaccinated with whole cell pertussis vaccines or with 6 different investigational acellular pertussis vaccines. Expression of this fragment should be useful for investigation of human serologic responses and construction of more effective pertussis vaccines.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 384
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None